Generation of an induced pluripotent stem cell line from a Chinese Han child with arrhythmia


CTNNA3, first reported in association with arrhythmogenic right ventricular cardiomyopathy in 2003, is an unique component of both desmosomes and adherens junctions. Using Sendaivirus-mediated reprogramming, we generated an induced pluripotent stem cell (iPSC) line from the peripheral blood mononuclear cells of a child with arrhythmia. The iPSCs exhibited stable amplification, expressed pluripotent markers, and differentiated spontaneously into three germ layers in vitro. Additionally, this iPSC line was found to maintain a normal karyotype and retain the pathogenic mutation in CTNNA3, facilitating a platform to study the disease mechanisms of arrhythmia and dysfunctions related to CTNNA3 mutations. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Hou C, Liu W, Xie L, Zheng J, Chen X, Sun X, Zhang Y, Xu M, Li Y, Xiao T
Journal Stem cell research
Publication Date 2021 Mar;51:102183
PubMed 33497884
DOI 10.1016/j.scr.2021.102183

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