Generation of a serine/threonine-protein kinase LATS1 gene-edited iPSC MUSIi012-A-3


In mammals, there are a number of kinases, including serine/threonine-protein kinase LATS1, that act as a core kinase of the Hippo pathway and that negatively regulate the Hippo effector protein YAP and its paralog TAZ. Using CRISPR/Cas9 technology, we established a stable LATS1 knockdown (LATS1-KD) iPSC from the MUSIi012-A cell line. The LATS1-KD iPSC MUSIi012-A-3 that was developed maintained both the normal karyotype and the pluripotent phenotype, and retained the ability to differentiate into all three embryonic germ layers. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Lorthongpanich C, Laowtammathron C, Jiamvoraphong N, Srisook P, Chingsuwanrote P, Klaihmon P, Waeteekul S, U-Pratya Y, Issaragrisil S
Journal Stem cell research
Publication Date 2020 Oct;48:101950
PubMed 32791482
DOI 10.1016/j.scr.2020.101950

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