Generation of two homozygous SHOX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9
Summary
SHOX2 is a homeobox transcription factor associated with atrial fibrillation (AF) and sinus node dysfunction. Here, we generated two homozygous SHOX2 knock-out hiPSC lines from a healthy control line and a corrected AF patient line (disease-specific SHOX2 mutation corrected to WT) using CRISPR/Cas9. These cell lines maintained pluripotency, an ability to differentiate into all three germlayers and a normal karyotype, presenting a valuable tool to investigate the impact of a full SHOX2 knock-out with respect to arrhythmogenic diseases on a cellular level. Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Rädecke K, Gore A, Burau K, Laugsch M, Köhler K, Rappold GA, Hoffmann S |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Jun;69:103089 |
| PubMed | 37028180 |
| DOI | 10.1016/j.scr.2023.103089 |