DPEDi001-A-1

General

Cell Line

hPSCreg Name
DPEDi001-A-1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 17th February 2023
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Provider

Generator Institute of Human Genetics Heidelberg (UKHG)

External Databases

BioSamples SAMEA112657099
CLO CLO_0104327

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA112625001

Ethics

Also have a look at the ethics information for the parental line DPEDi001-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line DPEDi001-A.

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Is the used vector excisable?
Yes
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Medium Other medium:
Base medium: StemFit
Main protein source:
Serum concentration: %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Atrial Fibrillation
Synonyms
  • AFib
  • AF
  • Atrial fibrillation
  • Atrial Fibrillation
show more synonyms
Genetic modifications
SHOX2 (target)
Gene knock-out
CRISPR-associated (CRISPR/Cas) System