DPEDi001-A-1
General
Cell Line |
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| hPSCreg Name | DPEDi001-A-1 |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 17th February 2023 |
| User feedback | |
Provider |
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| Generator | Institute of Human Genetics Heidelberg (UKHG) |
External Databases |
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| BioSamples | SAMEA112657099 |
| CLO | CLO_0104327 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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External Databases (Donor) |
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| BioSamples | SAMEA112625001 |
Ethics
Also have a look at the ethics information for the parental line
DPEDi001-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
DPEDi001-A.
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Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus (Lentivirus) |
| Is the used vector excisable? |
Yes |
| Absence of reprogramming vector(s)? |
Unknown |
| Reprogramming vectors silenced? | |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Medium |
Other medium:
Base medium: StemFit
Main protein source: Serum concentration: % |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| SOX2 |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XY
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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