Lymphoblast-derived hiPS cell lines generated from four individuals of a family of genetically unrelated parents and their female monozygotic twins


Lymphoblast cells from four individuals of a family of two genetically unrelated parents and their monozygotic twins were used to generate integration-free human induced pluripotent stem cells (hiPSCs). Reprogramming factors were delivered by co-electroporation of three episomal-based plasmids expressing OCT3/4, SOX2, KLF4, L-MYC and LIN28. The hiPSCs showed a normal karyotype, expressed pluripotency-associated markers, displayed the capacity for in vitro differentiation into the three germ layers and were Epstein Barr virus-free. These hiPSC lines offer the possibility to compare genetically unrelated and genetically identical tissues from different individuals and to study genotype-specific effects, which are particularly relevant for toxicology testing. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Bouma MJ, Freund C, IJzerman AP, Boomsma DI, Mummery CL, Raymond K
Journal Stem cell research
Publication Date 2019 Dec;41:101654
PubMed 31734644
DOI 10.1016/j.scr.2019.101654

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