Generation of human induced pluripotent stem cell (iPSC) lines (UUMCBi001-A, UUMCBi002-A) from two healthy donors


Availability of numerous high-quality iPSC lines is needed to overcome donor-associated variability caused by genetic background effects. We generated two human iPSC lines from dermal fibroblasts of two healthy females using Sendai virus reprogramming. Quality assessment of the iPSC lines confirmed the expression of pluripotency markers, trilineage differentiation capacity and absence of exogenous expression of reprogramming factors. Both iPSC lines were genetically stable with a genotype that matched the fibroblast lines of donors. These iPSC lines add to available reference lines as a resource for disease modeling of polygenic and multifactorial diseases, for evaluation of differentiation protocols and toxicology screening. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Vasylovska S, Schuster J, Brboric A, Carlsson PO, Dahl N, Lau J
Journal Stem cell research
Publication Date 2020 Dec 9;50:102114
PubMed 33340796
DOI 10.1016/j.scr.2020.102114

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