Generation of two induced pluripotent stem cell lines (UQACi002-A and UQACi005-A) from two patients with KRT14 epidermolysis bullosa simplex mutations


More than 107 pathogenic variations were identified in Keratin 14 gene (KRT14) in patients affected by epidermolysis bullosa simplex (EBS), a rare skin disease with still no curative treatment. Disease models as human induced pluripotent stem cells (hiPSCs) are promising tool for further advance the knowledge about this disorder and accelerate therapies development. Here, two hiPSC lines were reprogrammed from skin fibroblasts of two EBS patients carrying mutations within KRT14 by using CytoTune®Sendai virus. These iPSCs display pluripotent cell morphology, pluripotent markers expression, and the capability to differentiate into the three germ layers. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Bchetnia M, Martineau L, Racine V, Powell J, McCuaig C, Morin C, Dupérée A, Gros-Louis F, Laprise C
Journal Stem cell research
Publication Date 2022 May;61:102750
PubMed 35334406
DOI 10.1016/j.scr.2022.102750

Research Projects

Cell Lines