Generation of an integration-free iPSC line (CSCRi005-A) from erythroid progenitor cells of a healthy Indian male individual
Summary
Reprogramming of somatic cells with higher genome integrity, and use of non-integrating gene delivery methods and xeno-free cell culture conditions aid in the generation of iPSCs which are more suitable for disease modelling and clinical applications. We describe here an iPSC line generated using such conditions, which expressed all the pluripotency markers, retained normal karyotype and exhibited the potential for tri-lineage differentiation, both in-vitro and in-vivo. This is the first iPSC line available from a healthy Indian individual for researchers. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Manian KV, Bharathan SP, Maddali M, Srivastava VM, Srivastava A, Velayudhan SR |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2018 May;29:148-151 |
| PubMed | 29665501 |
| DOI | 10.1016/j.scr.2018.03.020 |