Generation of a double insulin and somatostatin reporter line, SCSe001-A-3, for the advancement of stem cell-derived pancreatic islets
Remarkable strides have been made over the past decade on the development of pancreatic β-cells from human stem cells through directed differentiation, allowing for modeling of β-cell development, function and disease. However, in vitro models and future therapeutic applications will require the use of stem cell-derived islets with multiple monohormonal endocrine cells types, including α, β, and δ cells. Using the previously reported Mel1 InsGFP/w human embryonic stem cell (hESC) line, we have knocked-in Red Fluorescence Protein (RFP) under the control of the endogenous somatostatin promoter using CRISPR/Cas9, generating a dual insulin and somatostatin reporter hESC line. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
|Authors||Leavens KF, Liao CM, Gagne AL, Kishore S, Cardenas-Diaz FL, French DL, Gadue P|
|Journal||Stem cell research|
|Publication Date||2020 Dec 8;50:102112|