MEL-1

General#

Cell Line

hPSCreg Name SCSe001-A
Alternative name(s)
MEL-1
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator Stem Cell Sciences plc (SCS)
Derivation country United Kingdom

External Databases

BioSamples SAMEA7783168
Cellosaurus CVCL_B863
NIHhESC NIHhESC-11-0139

General Information

Publications
Projects
Is the cell line available in principle?
No
Subclones

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA7782418

Ethics#

We are currently revising this section.

hESC Derivation#

Embryo stage Morula
Cell isolation Immunosurgery

Culture Conditions#

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
Medium Other medium:
Base medium: High glucose DMEM
Main protein source: Fetal Calf Serum
Serum concentration: 20 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
TDGF1
Yes
Alkaline Phosphatase
Yes
SSEA-3
No
TRA 1-60
Yes
TRA 1-81
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)