MEL-1
SCSe001-A
General#
Cell Line |
|
| hPSCreg Name | SCSe001-A |
| Alternative name(s) |
MEL-1
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| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 28th July 2017 |
| User feedback | |
Provider |
|
| Generator |
Stem Cell Sciences plc (SCS)
Contact:
Embryonic Stem (ES) Cell Biology |
| Derivation country | United Kingdom |
External Databases |
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| BioSamples | SAMEA7783168 |
| Cellosaurus | CVCL_B863 |
| NIHhESC | NIHhESC-11-0139 |
General Information |
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| Publications |
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| Projects | |
| Is the cell line available in principle? |
No |
| Subclones | |
Donor Information#
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA7782418 |
Ethics#
We are currently revising this section.hESC Derivation#
| Embryo stage | Morula |
| Cell isolation | Immunosurgery |
Culture Conditions#
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
| Passage method | Mechanically |
| Medium |
Other medium:
Base medium: High glucose DMEM
Main protein source: Fetal Calf Serum Serum concentration: 20 % |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| DNMT3B |
Yes |
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| GABRB3 |
Yes |
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| GDF3 |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| SOX2 |
Yes |
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| TDGF1 |
Yes |
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| Alkaline Phosphatase |
Yes |
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| SSEA-3 |
No |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
|
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XY
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Other Genotyping (Cell Line) |
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