MEL-1
SCSe001-A
General#
Cell Line |
|
hPSCreg Name | SCSe001-A |
Alternative name(s) |
MEL-1
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Cell line type | Human embryonic stem cell (hESC) |
Last update | 28th July 2017 |
User feedback | |
Provider |
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Generator |
Stem Cell Sciences plc (SCS)
Contact:
Embryonic Stem (ES) Cell Biology |
Derivation country | United Kingdom |
External Databases |
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BioSamples | SAMEA7783168 |
Cellosaurus | CVCL_B863 |
NIHhESC | NIHhESC-11-0139 |
General Information |
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Publications |
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Projects | |
Is the cell line available in principle? |
No |
Subclones |
Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
|
Diseases | No disease was diagnosed.
|
External Databases (Donor) |
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BioSamples | SAMEA7782418 |
Ethics#
We are currently revising this section.hESC Derivation#
Embryo stage | Morula |
Cell isolation | Immunosurgery |
Culture Conditions#
Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
Passage method | Mechanically |
Medium |
Other medium:
Base medium: High glucose DMEM
Main protein source: Fetal Calf Serum Serum concentration: 20 % |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
DNMT3B |
Yes |
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GABRB3 |
Yes |
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GDF3 |
Yes |
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NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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ZFP42 (REX-1) |
Yes |
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SOX2 |
Yes |
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TDGF1 |
Yes |
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Alkaline Phosphatase |
Yes |
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SSEA-3 |
No |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46XY
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Other Genotyping (Cell Line) |
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