MEL-1

General#

Cell Line

hPSCreg Name SCSe001-A
Alternative name(s)
MEL-1
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
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Provider

Generator Stem Cell Sciences plc (SCS)
Derivation country United Kingdom

External Databases

BioSamples SAMEA7783168
Cellosaurus CVCL_B863
NIHhESC NIHhESC-11-0139

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
No
Subclones

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA7782418

Ethics#

Was the embryo established purely for research purposes? Yes
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Alternatives to consent are available? Yes
Alternatives to consent
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? Yes
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved?
Approval number

hESC Derivation#

Embryo stage Morula
Cell isolation Immunosurgery

Culture Conditions#

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
Medium Other medium:
Base medium: High glucose DMEM
Main protein source: Fetal Calf Serum
Serum concentration: 20 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
TDGF1
Yes
Alkaline Phosphatase
Yes
SSEA-3
No
TRA 1-60
Yes
TRA 1-81
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)