Generation of RAB39B knockout isogenic human embryonic stem cell lines to model RAB39B-mediated Parkinson's disease


Mutations in RAB39B are a known cause of X-linked early onset Parkinson's disease. Isogenic human embryonic stem cell lines carrying two independent deletions of RAB39B were generated using CRISPR/Cas9 genome editing tool. The deletions were confirmed by PCR and direct sequence analysis in two edited stem cell lines. Both cell lines showed pluripotency and displayed a normal karyotype. Further, they were able to form embryoid bodies in vitro, and express markers indicative of differentiation to the three germ layers. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Gao Y, Wilson GR, Bozaoglu K, Elefanty AG, Stanley EG, Dottori M, Lockhart PJ
Journal Stem cell research
Publication Date 2018 Apr;28:161-164
PubMed 29499499
DOI 10.1016/j.scr.2018.02.015

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