GENEA029
GENEAe014-A
General#
Cell Line |
|
| hPSCreg Name | GENEAe014-A |
| Alternative name(s) |
GENEA029
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| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 10th April 2017 |
| User feedback | |
Provider |
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| Generator |
Genea (GENEA)
Contact:
Biocells |
| Owner | Biocells |
| Distributors | |
External Databases |
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| BioSamples | SAMEA104012532 |
| Cellosaurus | CVCL_9038 |
General Information |
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| Publications | |
| Is the cell line available in principle? | Available, without restrictions |
Donor Information#
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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External Databases (Donor) |
|
| BioSamples | SAMEA104012533 |
Ethics#
We are currently revising this section.hESC Derivation#
| Date of derivation | 2009-02-24 |
| Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
| PGD Embryo? |
No |
Culture Conditions#
| Medium |
Other medium:
Base medium: DMEM/F-12
Main protein source: Fetal bovine serum Serum concentration: 20 % Supplements
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
Teratoma positive, Alkaline Phosphatase stain positive.
Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46, XX no abnormalities detected
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Other Genotyping (Cell Line) |
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