A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells

Summary

We used successfully human foreskin fibroblasts as feeder cells for derivation and continued undifferentiated growth of hES cells. These feeder cells are convenient for IVF units, because no fetal human tissues or tissue from operations are needed.

Authors Hovatta O, Mikkola M, Gertow K, Strömberg AM, Inzunza J, Hreinsson J, Rozell B, Blennow E, Andäng M, Ahrlund-Richter L
Journal Human reproduction (Oxford, England)
Publication Date 2003 Jul;18(7):1404-9
PubMed 12832363
DOI 10.1093/humrep/deg290

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