HT852-DC
NIHTVBi019-A-1
General
Cell Line |
|
| hPSCreg name | NIHTVBi019-A-1 |
| Cite as: | NIHTVBi019-A-1 (RRID:CVCL_B7SW) |
| Alternative name(s) |
HT852-DC
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
PUFHi004-A (TMEM163 c.227T>C p.(Leu76Pro) iPSC) Donor's gene variants: TMEM 163 Donor diseases: Leukodystrophy |
| Last update | 11th April 2022 |
| User feedback | |
Provider |
|
| Generator | National Institutes of Health-National Heart, Lung, and Blood Institute (NIHTVB) |
| Owner | National Institutes of Health-National Heart, Lung, and Blood Institute (NIHTVB) |
| Distributors | |
| Derivation country | United States |
External Databases |
|
| BioSamples | SAMEA14027204 |
| Cellosaurus | CVCL_B7SW |
| Wikidata | Q114312530 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | female |
| Ethnicity | Caucasian/White |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Yes
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA14026314 |
Ethics
Also have a look at the ethics information for the parental line
NIHTVBi019-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
|
|
The source cell information can be found in the parental cell line
NIHTVBi019-A.
|
|
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
Vector free reprogramming |
|
| Type of used vector free reprogramming factor(s) |
None
|
Other |
|
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Medium |
Essential 8™
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
|
||||
| SSEA-4 |
Yes |
|
||||
| TRA 1-60 |
Yes |
|
Differentiation Potency
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Disease/phenotype related modifications |
|
Login to share your feedback, experiences or results with the research community.