NIHTVBi019-A-1

HT852-DC

General

Cell Line

hPSCreg Name
NIHTVBi019-A-1
Alternative name(s)
HT852-DC
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 11th April 2022
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Provider

Generator National Institutes of Health-National Heart, Lung, and Blood Institute (NIHTVB)
Owner National Institutes of Health-National Heart, Lung, and Blood Institute (NIHTVB)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA14027204
CLO CLO_0103881
Cellosaurus CVCL_B7SW

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian/White

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Leukodystrophy
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Leukodystrophy

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
G-Banding
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA14026314

Ethics

Also have a look at the ethics information for the parental line NIHTVBi019-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line NIHTVBi019-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Medium Essential 8™

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
AFP
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
HAND1
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
TUBB3
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
G-banding
Passage number: 41
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Leukodystrophy
Synonyms
  • Leukodystrophy
Genetic modifications
bloc1s1 (target)
Isogenic modification
Heterozygous
two point mutations have been "normalized"
Repaired