Generation of heterozygous (MRli003-A-1) and homozygous (MRli003-A-2) MYH10 knockout human iPSC lines


Myosin-10, also known as non-muscle myosin IIB, is a cytoskeletal protein implicated in cardiac development and disease. In humans, it is encoded by the MYH10 gene. Using CRISPR/Cas9 gene editing technology, we generated two MYH10 knockout human iPSC lines - one heterozygous (MRli003-A-1) and one homozygous (MRli003-A-2) - by introducing a frameshift deletion in exon 2. We then verified that both lines had maintained pluripotency, parental cell morphology, trilineage differentiation potential and a normal karyotype. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Zhang F, Meier AB, Laugwitz KL, Dorn T, Moretti A
Journal Stem cell research
Publication Date 2021 Nov 29;57:102612
PubMed 34864222
DOI 10.1016/j.scr.2021.102612

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