Generation of RUNX1-null reporter human embryonic stem cell line GIBHe008-A


RUNX1/AML1/CBFA2 (runt-related transcription factor 1/acute myeloid leukemia 1 protein/core-binding factor subunit alpha-2), is a transcription factor that plays a critical role in the development of normal hematopoiesis. RUNX1 is also essential for the development of immune cells and sensory neurons. Chromosomal translocations involving the gene have been associated with several types of leukemia. To investigate the role of RUNX1 in human hematopoietic development we generated RUNX1-null human embryonic stem cell reporter line GIBHe008-A by TALEN mediated homologous recombination. This cell line GIBHe008-A was subjected to detailed characterization by standard assays for human pluripotent stem cells. It provides an ideal model to study the role of RUNX1 in the hESC-derived developmental models. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Ullah H, You H, Shah Z, Fan C, Zhang B, Liu H, Zhang J, Abbas N, Filonenko ES, Samokhvalov IM
Journal Stem cell research
Publication Date 2020 May;45:101800
PubMed 32371361
DOI 10.1016/j.scr.2020.101800

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