RASe003-A

Registration Summary:

A P E C

ESM03

RASe003-A

General#

Cell Line
hPSCreg Name	RASe003-A
Alternative name(s)	ESM03
Cell line type	Human embryonic stem cell (hESC)
Last update	22nd May 2019
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Provider
Generator	Russian Academy of Sciences (RAS) Contact: Russian Academy of Sciences (RAS)
Distributors	European Collection of Authenticated Cell Cultures (ECACC)
External Databases
Cellosaurus	CVCL_C645
CLO	CLO_0100235
Wikidata	Q54882913
General Information
Publications	Lagarkova MA et al. Induction of pluripotency in human endothelial cells resets epigenetic profile on genome scale. Cell cycle (Georgetown, Tex.). 2010 Mar 1;9(5):937-46. Lagarkova MA et al. Human embryonic stem cell lines isolation, cultivation, and characterization. In vitro cellular & developmental biology. Animal. 2010 Apr;46(3-4):284-93. Panova AV et al. Epigenetic reprogramming by naïve conditions establishes an irreversible state of partial X chromosome reactivation in female stem cells. Oncotarget. 2018 May 18;9(38):25136-25147.
* Is the cell line readily obtainable for third parties?	No

Donor Information#

General Donor Information
Phenotype and Disease related information (Donor)
Diseases

Ethics#

Alternatives to consent

hESC Derivation#

Culture Conditions#

Passage method

Mechanically

Characterisation#

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	FACS	Enzymatic Assay	Expression Profiles
DPPA3	Yes
Dppa5	Yes
NANOG	Yes
POU5F1 (OCT-4)	Yes

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vivo teratoma

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vivo teratoma

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vivo teratoma

Genotyping#

Karyotyping (Cell Line)
Other Genotyping (Cell Line)