KSCBi005-A-8(hiPSC-PD-L1KO), a PD-L1 knockout human induced pluripotent stem cell line for demonstrating the role of the PD-1/PD-L1 axis
Summary
Programmed Cell Death Ligand 1 (PD-L1) is a trans-membrane protein that attenuates the host immune response to tumor cells. PD-L1 ligand on the surface of the cancer binds to PD-1 transmembrane receptors on T cells and exhausts T cells function. Using CRISPR-Cas9 genome editing, we generated biallelic PD-L1 mutants in human induced pluripotent stem cells (hiPSCs). The PD-L1 homozygous-knockout hiPSCs retained their normal morphology, gene expression, and in vivo differentiation potential. Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Han HJ, Kim JH |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Oct;72:103196 |
| PubMed | 37690432 |
| DOI | 10.1016/j.scr.2023.103196 |