Generation of a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-2)
Summary
NK homeobox 1 (NKX2.1; also known as thyroid transcription factor 1, TTF-1) encodes for a transcription factor involved in the development of thyroid, lung and brain. Here, we established a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (iPSCs) using TALEN technology. The reporter enables the optimization and monitoring of the differentiation of pluripotent stem cells (PSCs) into NKX2.1 expressing cells on a single cell level, as well as the enrichment of NKX2.1 positive cells. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Olmer R, Dahlmann J, Merkert S, Baus S, Göhring G, Martin U |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2019 Aug;39:101492 |
| PubMed | 31299562 |
| DOI | 10.1016/j.scr.2019.101492 |