Generation of an induced pluripotent stem cell line (ZZUi034-A) from a 65 year old Chinese female donor with sendai virus reprogramming protocol


Peripheral blood was extracted from a 65 year old Chinese female health donor. Induced pluripotent stem cells (iPSC) were reprogrammed with the 4 reprogramming factors: Klf-4, c-Myc, Oct-4, Sox-2, using sendai virus reprogramming protocol. The iPSC line showed pluripotency which was confirmed by immunofluorescent staining; the iPSC line was also able to form embryoid bodies in vitro and differentiate into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This healthy iPSC line can be served as healthy control for disease mechanism and disease modeling study. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Zhang Y, Zhu J, Dai Y, Wang L, Liu R, Guo X
Journal Stem cell research
Publication Date 2022 Apr 19;62:102788
PubMed 35462158
DOI 10.1016/j.scr.2022.102788

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