FINCBi001-A

F56Lcl33, F56L 33M

General

Cell Line

hPSCreg name FINCBi001-A
Cite as:
FINCBi001-A (RRID:CVCL_YD66)
Alternative name(s)
F56Lcl33, F56L 33M
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
FINCBi006-A
(mt1108 clone #121, mt1108 #121)
Donor's gene variants:
MT-ND6, MT-ND6, MT-ND4L, MT-ND4L
Donor diseases:
Leber hereditary optic neuropathy
FINCBi005-A
(mt1072 clone #103, mt1072 #103)
Donor's gene variants:
MT-ND6, MT-ND6, MT-ND4L, MT-ND4L
Donor diseases:
Leber hereditary optic neuropathy
Last update 3rd April 2024
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Fondazione IRCCS Istituto Neurologico C. Besta (FINCB)
Owner Fondazione IRCCS Istituto Neurologico C. Besta (FINCB)
Derivation country Italy

External Databases

Cellosaurus CVCL_YD66
Wikidata Q93553991
BioSamples SAMEA115464453

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • LHON
  • Leber optic atrophy
Genetic variants
MT-ND1 (target)
Chromosome MT: 3,307-4,262 forward strand
NC_012920.1:m.3460G>A
m.3460G>A is a variant on mitochondrial DNA and is homoplasmic on donor fibroblasts.
Disease associated phenotypes
  • central vision loss
  • loss of retinal ganglion cells
  • optic atrophy

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA115464456

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Dr Valerio Carelli, IRCCS Istituto delle Scienze Neurologiche di Bologna
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee of Fondazione IRCCS Istituto Neurologico Carlo Besta, Milan, Italy
Approval number n.43, 26th June 2013
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast
  • FIBROBLAST
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™ Flex
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
SOX2
Yes
ZFP42 (REX-1)
Yes
Alkaline Phosphatase
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
FOXA2
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
MSX1
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
normal male karyotype (46,XX)
Karyotyping method: Array CGH

Other Genotyping (Cell Line)