FINCBi005-A

mt1072 clone #103, mt1072 #103

General

Cell Line

hPSCreg name FINCBi005-A
Cite as:
FINCBi005-A (RRID:CVCL_D6I4)
Alternative name(s)
mt1072 clone #103, mt1072 #103
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
FINCBi006-A
(mt1108 clone #121, mt1108 #121)
Donor's gene variants:
MT-ND6, MT-ND6, MT-ND4L, MT-ND4L
Donor diseases:
Leber hereditary optic neuropathy
FINCBi001-A
(F56Lcl33, F56L 33M)
Donor's gene variants:
MT-ND1, MT-ND1
Donor diseases:
Leber hereditary optic neuropathy
Last update 3rd April 2024
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Provider

Generator Fondazione IRCCS Istituto Neurologico C. Besta (FINCB)
Owner Fondazione IRCCS Istituto Neurologico C. Besta (FINCB)
Derivation country Italy

External Databases

BioSamples SAMEA115082457
Cellosaurus CVCL_D6I4

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:

cell line can be obtained by third parties using appropriate MTA

Donor Information

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • LHON
  • Leber optic atrophy
Genetic variants
MT-ND6 (target)
Chromosome MT: 14,149-14,673 reverse strand
NC_012920.1:m.14484T>C
m.14484T>C is a variant on mitochondrial DNA and is 70% heteroplasmic on donor fibroblasts.
MT-ND4L (target)
Chromosome MT: 10,470-10,766 forward strand
NC_012920.1:m.10680G>A
m.10680G>A is a variant on mitochondrial DNA and is 70% heteroplasmic on donor fibroblasts.
Disease associated phenotypes
  • central vision loss
  • loss of retinal ganglion cells
  • optic atrophy

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA115082456

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Dr. Costanza Lamperti, Fondazione IRCCS Istituto Neurologico Carlo Besta
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee of Fondazione IRCCS Istituto Neurologico Carlo Besta, Milan, Italy
Approval number n.60, 6th March 2019
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast
  • FIBROBLAST
show more synonyms
Passage number reprogrammed p3

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™ Flex
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
NANOG
Yes
SOX2
Yes
ZFP42 (REX-1)
Yes
Alkaline Phosphatase
Yes
DPPA4
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
FOXA2
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
MSX1
Yes
TBXT
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
PAX6
Yes
SOX1
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
normal male karyotype (46,XY)
Passage number: p7
Karyotyping method: Array CGH

Other Genotyping (Cell Line)