Generation and characterization of induced pluripotent stem cell line IITGi001-A derived from adult human primary dermal fibroblasts


Adult human primary dermal fibroblasts [ATCC (PCS-201-012)] were reprogrammed by transfection of oriP/EBNA-1 based episomal plasmids expressing OCT3/4, SOX2, KLF4, L-MYC, LIN28 and a p53 shRNA (Okita et al., 2011) to give rise to induced pluripotent stem cells (iPSCs). These iPSCs expressed core pluripotency markers, maintained normal karyotype, and showed tri-lineage differentiation potential. Further, the absence of episomal plasmid integration in this iPSC line was confirmed by genomic PCR. In addition, DNA fingerprinting of fibroblast and iPSC DNA by microsatellite analysis confirmed the genetic identity of this cell line. This iPSC line was shown to be free from mycoplasma contamination. Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Raina K, Joshi G, Modak K, Premkumar C, Priyanka S, Rajesh P, Velayudhan SR, Thummer RP
Journal Stem cell research
Publication Date 2023 Sep;71:103159
PubMed 37392703
DOI 10.1016/j.scr.2023.103159

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