Generation of induced pluripotent stem cell line (RCMGi008-A) from human skin fibroblasts of a cystic fibrosis patient with compound heterozygous F508del/CFTRdele2.3 mutations in CFTR gene


Skin fibroblasts obtained from a 20-year-old woman with clinically manifested and genetically proven (F508del/CFTRdele2.3) cystic fibrosis were successfully transformed into induced pluripotent stem cells (iPSCs) by using Sendai virus-based reprogramming vectors including the four Yamanaka factors, OCT3/4, SOX2, KLF4, and c-MYC. The iPSCs showed a normal karyotype, expressed pluripotency markers and exhibited the potential to differentiate into three germ layers in spontaneous differentiation assay. This iPSC line may be used for development of a personalized treatment including genome editing, disease modelling, cell differentiation and organoid formation, pharmacological investigations and drug screening. Copyright © 2022. Published by Elsevier B.V.

Authors Kondrateva E, Panchuk I, Demchenko A, Grigorieva O, Zheglo D, Voronina E, Erofeeva A, Tabakov V, Orlova M, Lavrov A, Smirnikhina S, Kutsev S
Journal Stem cell research
Publication Date 2022 Aug;63:102854
PubMed 35843019
DOI 10.1016/j.scr.2022.102854

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