ATLABi001-A

AT-MODY3-iP001

General#

Cell Line

hPSCreg Name
ATLABi001-A
Alternative name(s)
AT-MODY3-iP001
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 30th September 2021
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Provider

Generator IMCB, Adrian Teo's Lab (ATLAB)
Owner IMCB, Adrian Teo's Lab (ATLAB)
Distributors
Derivation country Singapore

External Databases

BioSamples SAMEA9726670
CLO CLO_0103512

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 10-14
Ethnicity Asian Chinese

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Hepatocyte Nuclear Factor 1-Alpha-Associated Monogenic Diabetes (primary disease)
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Maturity-Onset Diabetes of the Young, Type 3
  • HNF1A-Associated Monogenic Diabetes
  • Hepatocyte Nuclear Factor 1-Alpha-Associated Monogenic Diabetes
  • MODY3
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46, XX
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

All cell lines of this donor's relatives
Has sister:

External Databases (Donor)

BioSamples SAMEA10201990

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: ateo@imcb.a-star.edu.sg
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink Dr. Adrian Teo : ateo@imcb.a-star.edu.sg
Alternatives to consent are available? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? NHG DSRB
Approval number 2013/01068
Do you have obligations to third parties in regard to the use of the cell line? Yes
Please describe: In consultation with clinician collaborator
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
skin fibroblast
Age of donor (at collection) 10-14

Reprogramming method

Vector type Non-integrating
Vector Episomal

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Feeder cells Irradiated CF-1 mouse embryonic fibroblasts
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
HEPES 15 mM
L-glutamine
NEAA
FGF2 10 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Unknown
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Unknown

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
UBERON_0000924
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)