FA3

General

Cell Line

hPSCreg name UTSWi003-A
Cite as:
UTSWi003-A
Alternative name(s)
FA3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UTSWi003-A-1
(FA3ic)
Donor's gene variants:
fxn
Donor diseases:
Friedreich Ataxia
UTSWi003-A-2
(FAN3)
Donor's gene variants:
fxn
Donor diseases:
Friedreich Ataxia
UTSWi003-A-3
(FAN3ic)
Donor's gene variants:
fxn
Donor diseases:
Friedreich Ataxia
UTSWi001-A-1
(FA1ic)
Donor diseases:
Friedreich Ataxia
UTSWi002-A-1
(FA2ic)
Donor diseases:
Friedreich Ataxia
UTSWi001-A
(FA1)
Donor diseases:
Friedreich Ataxia
USFi001-A
(1CN1.5)
Donor diseases:
Friedreich Ataxia
UTSWi002-A
(FA2)
Donor diseases:
Friedreich Ataxia
IGIBi012-A
(FA-hiPSC_001)
Donor diseases:
Friedreich Ataxia
IGIBi013-A
(FA_hiPSC_002)
Donor diseases:
Friedreich Ataxia
IGIBi016-A
(FA-hiPSC_005)
Donor diseases:
Friedreich Ataxia
UTSWi001-A-2
(FAN1)
Donor diseases:
Friedreich Ataxia
UTSWi001-A-3
(FAN1ic)
Donor diseases:
Friedreich Ataxia
UTSWi002-A-2
(FAN2)
Donor diseases:
Friedreich Ataxia
UTSWi002-A-3
(FAN2ic)
Donor diseases:
Friedreich Ataxia
BBANTWi007-A
(iPSC_BrS10_FB_C3)
Donor's gene variants:
SCN5A
Donor diseases:
Brugada syndrome 1
LUMCi027-A
(LUMC0153iPKP03)
Donor's gene variants:
PKP2
Donor diseases:
arrhythmogenic right ventricular dysplasia 9
RCMGi002-A
(P7L2)
Donor diseases:
Cystic Fibrosis
LUEi017-A
(iPS-L-2122)
Donor diseases:
Parkinson Disease
LUEi018-A
(iPS-L-2124)
Donor diseases:
Parkinson Disease
RCMGi008-A
(P9L1)
Donor diseases:
Cystic Fibrosis
RCMGi007-A
(P8L2)
Donor diseases:
Cystic Fibrosis
LUMCi030-B
(LUMC0110iALK10)
Donor diseases:
Hereditary Hemorrhagic Telangiectasia
RCMGi004-A
(P6L2)
Donor diseases:
Cystic Fibrosis
BBANTWi006-A
(iPSC_BrS9_FB_C7)
Donor's gene variants:
SCN5A
Donor diseases:
Brugada syndrome 1
RCMGi013-A
(P2L2)
Donor's gene variants:
CFTR
Donor diseases:
Cystic Fibrosis
RCMGi005-A
(P5L1)
Donor diseases:
Cystic Fibrosis
RCMGi001-A
(P1L5)
Donor diseases:
Cystic Fibrosis
LUEi014-A
(iPS-B-125)
Donor diseases:
Parkinson Disease
LUEi015-A
(iPS-B-11)
Donor diseases:
Parkinson Disease
TAUi006-A
(UTA.00102.LQT1)
Donor's gene variants:
KCNQ1 – potassium voltage-gated channel subfamily Q member 1
Donor diseases:
Long QT Syndrome 1
RCMGi005-B
(P5L5)
Donor diseases:
Cystic Fibrosis
TAUi006-B
(UTA.00118.LQT1)
Donor's gene variants:
KCNQ1 – potassium voltage-gated channel subfamily Q member 1
Donor diseases:
Long QT Syndrome 1
TAUi007-A
(UTA.00208.LQT1)
Donor's gene variants:
KCNQ1 – potassium voltage-gated channel subfamily Q member 1
Donor diseases:
Long QT Syndrome 1
TAUi007-B
(UTA.00211.LQT1)
Donor's gene variants:
KCNQ1 – potassium voltage-gated channel subfamily Q member 1
Donor diseases:
Long QT Syndrome 1
LUMCi030-A
(LUMC0110iALK04)
Donor diseases:
Hereditary Hemorrhagic Telangiectasia
RCMGi004-B
(P6L8)
Donor diseases:
Cystic Fibrosis
Last update 25th March 2024
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Provider

Generator University of Texas Southwestern Medical Center (UTSW)
Derivation country United States

External Databases

BioSamples SAMEA115429408

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: allowed
Subclones

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Friedreich's Ataxia
  • Friedreich Ataxia
  • Friedreich ataxia
Genetic variants
fxn (target)
Is the medical history available upon request? limited
Is clinical information available? limited

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

External Databases (Donor)

BioSamples SAMEA115429409

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Dr. David Lynch, CHOP
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink Dr. David Lynch
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? No
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? CHOP IRB
Approval number 10-007864
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? CHOP IRB
Approval number 10-007864
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

Source cell type

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
Immunostaining, RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Dispase
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
foxa2
Yes
sox17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
tbxt2
Yes
ncam1
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
pax6
Yes
nestin
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)