General

Cell Line
hPSCreg name	UTSWi002-A-2
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	UTSWi002-A-2
Alternative name(s)	FA2-N
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	UTSWi002-A (FA2) Donor diseases: Friedreich Ataxia UTSWi002-A-1 (FA2ic) Donor diseases: Friedreich Ataxia UTSWi002-A-3 (FA2ic-N) Donor diseases: Friedreich Ataxia UTSWi001-A-1 (FA1ic) Donor diseases: Friedreich Ataxia UTSWi003-A-1 (FA3ic) Donor's gene variants: fxn Donor diseases: Friedreich Ataxia IGIBi012-A (FA-hiPSC_001) Donor diseases: Friedreich Ataxia IGIBi013-A (FA_hiPSC_002) Donor diseases: Friedreich Ataxia IGIBi016-A (FA-hiPSC_005) Donor diseases: Friedreich Ataxia UTSWi001-A (FA1) Donor diseases: Friedreich Ataxia USFi001-A (1CN1.5) Donor diseases: Friedreich Ataxia UTSWi001-A-3 (FA1ic-N) Donor diseases: Friedreich Ataxia UTSWi003-A (FA3) Donor's gene variants: fxn Donor diseases: Friedreich Ataxia UTSWi001-A-2 (FA1-N) Donor diseases: Friedreich Ataxia UTSWi003-A-2 (FA3-N) Donor's gene variants: fxn Donor diseases: Friedreich Ataxia UTSWi003-A-3 (FA3ic-N) Donor's gene variants: fxn Donor diseases: Friedreich Ataxia
Last update	4th June 2024
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Provider
Generator	University of Wollongong (UOW)
External Databases
BioSamples	SAMEA115432925
General Information
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed
Subclone of	UTSWi002-A

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Friedreich Ataxia The donor is a carrier of a disease-associated mutation and affected. Synonyms Friedreich's Ataxia Friedreich Ataxia Friedreich ataxia
Is the medical history available upon request?	limited
Is clinical information available?	limited
Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?	No
External Databases (Donor)
BioSamples	SAMEA115428748

Ethics

Also have a look at the ethics information for the parental line UTSWi002-A .

For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
The source cell information can be found in the parental cell line UTSWi002-A.
Reprogramming method
Vector type	Non-integrating
Vector	Sendai virus
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

Culture Conditions

Surface coating	Vitronectin
Feeder cells	No
Passage method	Enzyme-free cell dissociation EDTA
Medium	Essential 8™

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line UTSWi002-A .

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease

Type of modification Transgene expression Chromosome location 13q32.3 Free text This line expresses Neurogenin 2 under a doxycycline inducible promoter. The line also contains a floxed Blasticidin resistance/nuclear mApple selection cassette. Delivery method CRISPR-associated (CRISPR/Cas) System