WA09, H9


Cell Line

hPSCreg name CHOPe003-A
Cite as:
Alternative name(s)
WA09, H9
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 15th August 2023
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Generator The Children's Hospital of Philadelphia (CHOP)

External Databases

BioSamples SAMEA114268384
Cellosaurus CVCL_C9EC
Wikidata Q123030926

General Information

* Is the cell line readily obtainable for third parties?
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?

External Databases (Donor)

BioSamples SAMEA114268385


Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Wicell
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Alternatives to consent are available? Yes
Alternatives to consent NIH Registry Number 0062; US Stem Cell Bank Registry number SCSC06-18
Alternative consent approval number NIHhESC-10-0062
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? University of WI IRB
Approval number 95-623-239

hESC Derivation

Date of derivation 1998-01-01
Supernumerary embryos from IVF treatment?
Separation of research and IVF treatment?
PGD Embryo?

Culture Conditions

Medium Other medium:
Base medium: DMEMF12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %


Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Morphology pictures
Differentiation Potency
Ont Id: UBERON_0000925
In vitro directed differentiation
Ont Id: UBERON_0000926
In vitro directed differentiation
Ont Id: UBERON_0000924
In vitro directed differentiation

Certificate of Analysis

Is there a certificate of analysis available?
Passage: 30


Karyotyping (Cell Line)

Has the cell line karyotype been analysed?

Other Genotyping (Cell Line)