SIVF10, GENEA010
The cell line is not validated yet.
GENEAe009-A
General#
Cell Line |
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hPSCreg Name | GENEAe009-A |
Alternative name(s) |
SIVF10, GENEA010
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Cell line type | Human embryonic stem cell (hESC) |
Last update | 10th April 2017 |
User feedback | |
Provider |
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Generator |
Genea (GENEA)
Contact:
Biocells |
Derivation country | Australia |
External Databases |
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BioSamples | SAMEA104012416 |
Cellosaurus | CVCL_9019 |
General Information |
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Publications | |
Is the cell line available in principle? |
No |
Donor Information#
General Donor Information |
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Sex | female |
Phenotype and Disease related information (Donor) |
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Diseases | |
External Databases (Donor) |
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BioSamples | SAMEA104012415 |
Ethics#
We are currently revising this section.hESC Derivation#
Embryo stage | Blastula with ICM and Trophoblast |
Supernumerary embryos from IVF treatment? |
Yes
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PGD Embryo? |
Yes |
ZP removal technique | Enzymatic |
Cell isolation | Mechanical |
Cell seeding | Isolated ICM |
Culture Conditions#
Passage method | Mechanically | |||||||||
Medium |
Other medium:
Base medium: DMEM/F-12
Main protein source: Fetal bovine serum Serum concentration: 20 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
Alkaline Phosphatase |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-1 |
No |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46XX
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Other Genotyping (Cell Line) |
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