SIVF10, GENEA010
The cell line is not validated yet.
GENEAe009-A
General#
Cell Line |
|
| hPSCreg Name | GENEAe009-A |
| Alternative name(s) |
SIVF10, GENEA010
|
| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
|
| Generator |
Genea (GENEA)
Contact:
Biocells |
| Derivation country | Australia |
External Databases |
|
| BioSamples | SAMEA104012416 |
| Cellosaurus | CVCL_9019 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information#
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
External Databases (Donor) |
|
| BioSamples | SAMEA104012415 |
Ethics#
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
| Name of accrediting authority involved? |
| Approval number |
hESC Derivation#
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
Yes |
| ZP removal technique | Enzymatic |
| Cell isolation | Mechanical |
| Cell seeding | Isolated ICM |
Culture Conditions#
| Passage method | Mechanically | |||||||||
| Medium |
Other medium:
Base medium: DMEM/F-12
Main protein source: Fetal bovine serum Serum concentration: 20 % Supplements
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
|
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-1 |
No |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
|
Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XX
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Other Genotyping (Cell Line) |
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