HuES12

General#

Cell Line

hPSCreg Name HVRDe012-A
Alternative name(s)
HuES12
Cell line type Human embryonic stem cell (hESC)
Last update 7th October 2019
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Provider

Generator Harvard University (HVRD)
Contact:
Melton Lab
Derivation country United States

External Databases

Cellosaurus CVCL_B142
NIHhESC NIHhESC-09-0025

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics#

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Alternatives to consent are available? Yes
Alternatives to consent Please see the NIH hESC Registry https://grants.nih.gov/stem_cells/registry/current.htm?id=43 HUES 12 was given the NIH Approval # NIHhESC-09-0025
Alternative consent approval number
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
How may genetic information associated with the cell line be accessed?
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Harvard University Committee on the Use of Human Subjects/ Harvard ESCRO Committee
Approval number

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Expansion status 4
Stephenson 2007
ICM morphology A
Stephenson 2007
Trophectoderm morphology g
Stephenson 2007
Cell isolation Immunosurgery

Culture Conditions#

Feeder cells Mouse Embryonic Fibroblast Cell
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 10 %
Supplements
bFGF %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
TDGF1
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)