Generation of PRMT6 homozygous knockout human embryonic stem cell lines
PRMT6 is a type I protein arginine methyltransferase (PRMT) which participates in diverse biological processes. To facilitate the understanding of PRMT6 functions, we generated two independent homozygous PRMT6 knockout Human ESCs clones, PRMT6-KO-10 and PRMT6-KO-24, in human WA01 ES cells by CRISPR/Cas9. The pluripotency of both clones was verified by the presence of pluripotent markers, normal karyotypes, and differentiation potential in vivo whereas the lack of PRMT6 expression was demonstrated by sequencing and detection of the significant changes in specific histone methylation patterns. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.
|Li T, Jin W, He Y, Zhou Z
|Stem cell research
|2020 Dec 28;50:102136