Protocol for the generation of cultured cortical brain organoid slices
Summary
Three-dimensional brain organoids from human pluripotent stem cells are a powerful tool for studying human neural networks. Here, we present a protocol for generating cortical brain organoid slices (cBOS) derived from regionalized cortical organoids and grown at the air-liquid interphase. We provide steps for slicing organoids and maintaining them in long-term culture. We then detail approaches for quality control including the evaluation of cell death and cellular identity. Finally, we describe procedures for the expression of a genetically encoded nanosensor for ATP. For complete details on the use and execution of this protocol, please refer to Petersilie et al.1. Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.
| Authors | Petersilie L, Kafitz KW, Neu LA, Heiduschka S, Le S, Prigione A, Rose CR |
|---|---|
| Journal | STAR protocols |
| Publication Date | 2024 Sep 20;5(3):103212 |
| PubMed | 39128007 |
| PubMed Central | PMC11369419 |
| DOI | 10.1016/j.xpro.2024.103212 |