GM23338

General#

Cell Line

hPSCreg Name HVRDi004-B
Alternative name(s)
GM23338
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 28th September 2018
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Provider

Generator Harvard University (HVRD)
Distributors

External Databases

BioSamples SAMN00211020
Cellosaurus CVCL_F182

General Information

Publications
Is the cell line available in principle? Available, without restrictions
Subclones

Donor Information#

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMN02324437

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Age of donor (at collection) 55-59

Reprogramming method

Vector type Integrating
Vector Plasmid
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
Yes
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Feeder cells MEF
Medium Other medium:
Base medium: KO-DMEM (Invitrogen)
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
bFGF 4 ng/ml
β-ME
non-essential amino acid
Pen/Strep
L-Glutamine

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
POU5F1 (OCT-4)
Yes
NANOG
Yes
Transcriptome Characterisation
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Protocol or reference
journal.pgen.1000718.PDF
Lee et al. PLoS Genetics 2009
Bone Tissue
Ont Id: UBERON_0002481
In vivo teratoma
Protocol or reference
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
Protocol or reference

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Unkown

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes