ENCBS369AAA, iNGN

General#

Cell Line

hPSCreg Name HVRDi004-B-1
Alternative name(s)
ENCBS369AAA, iNGN
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 2nd October 2018
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Provider

Generator Harvard University (HVRD)
Owner Harvard Personal Genome Project
Derivation country United States

External Databases

BioSamples SAMN03485859

General Information

Publications
Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unkown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMN02324437

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

The source cell information can be found in the parental cell line HVRDi004-B.

Reprogramming method

Vector type Integrating
Vector Plasmid
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
Yes
Methods used
Sequencing

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
Differentiation Potency
Neuron
Ont Id: CL_0000540
In vitro directed differentiation
Marker Expressed
NEUROD1 neuronal differentiation 1
Yes
VGLUT1 solute carrier family 17 member 7
Yes
chAT choline O-acetyltransferase
Unkown
MAP2 microtubule associated protein 2
Unkown
Protocol or reference

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Unkown

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes

Genetic Modification#

Genetic modifications not related to a disease
Neurog1 Neurogenin 1 (target)
Transgene expression
Neurog 1 and Neurog 2 are expressed and regulated by Dox
Viral
Lentivirus