ENCBS369AAA, iNGN
The cell line is not validated yet.
HVRDi004-B-1
General
Cell Line |
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| hPSCreg name | HVRDi004-B-1 |
| Cite as: | HVRDi004-B-1 |
| Alternative name(s) |
ENCBS369AAA, iNGN
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
HVRDi004-B (GM23338) |
| Last update | 29th April 2022 |
| User feedback | |
Provider |
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| Generator |
Harvard University (HVRD)
Contact:
Personal Genome Project: Harvard |
| Owner | Harvard Personal Genome Project |
| Distributors | |
| Derivation country | United States |
External Databases |
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| BioSamples | SAMN03485859 |
General Information |
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| Publications | |
| Projects | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMN02324437 |
Ethics
Also have a look at the ethics information for the parental line
HVRDi004-B
.
| Is there an MTA available for the cell line? | Yes |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
HVRDi004-B.
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Reprogramming method |
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| Vector type | Integrating |
| Vector | Plasmid |
| Genes | |
| Is the used vector excisable? |
No |
| Absence of reprogramming vector(s)? |
No |
| Reprogramming vectors silenced? |
Yes |
| Methods used |
Sequencing
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Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Medium |
mTeSR™ 1
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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Differentiation Potency
In vitro directed differentiation
| Marker | Expressed |
| NEUROD1 neuronal differentiation 1 |
Yes |
| VGLUT1 solute carrier family 17 member 7 |
Yes |
| chAT choline O-acetyltransferase |
Unknown |
| MAP2 microtubule associated protein 2 |
Unknown |
Protocol or reference
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Certificate of Analysis |
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| Is there a certificate of analysis available? |
Yes
Passage:
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Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Unknown
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Other Genotyping (Cell Line) |
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| Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE60548
nCounter miRNA
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE62145
Agilent microarray
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE62146 |
Genetic Modification
| Genetic modifications not related to a disease |
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