Royan H3

General#

Cell Line

hPSCreg Name
RIe003-A
Alternative name(s)
Royan H3
Cell line type Human embryonic stem cell (hESC)
Last update 22nd March 2021
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Royan Institute (RI)
Distributors
Derivation country Iran

External Databases

Cellosaurus CVCL_9384
CLO CLO_0103256
Wikidata Q54950986

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information#

General Donor Information

Sex unknown

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Triploidy
The donor is affected.
Synonyms
  • Triploid
  • Triploidy
Disease associated phenotypes
  • Triploid Set of Chromosomes

Ethics#

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Expansion status 4
Stephenson 2007
ICM morphology A
Stephenson 2007
Trophectoderm morphology a
Stephenson 2007
ZP removal technique Chemical
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions#

Surface coating Gelatin
Feeder cells
Yes
Passage method Enzymatically and Mechanically
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Fetal Calf Serum
Serum concentration: 20 %
Supplements
L-Glutamine

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Connexin43
Yes
SSEA-1
No
Alkaline Phosphatase
Yes
nodal
Yes
ZFP42 (REX-1)
Yes
LEFTY1
Yes
foxd3
Yes
Cripto
Yes
CXCR4
Yes
EPHA2
Yes
FGF4
Yes
NANOG
Yes
PAX6
Yes
SOX2
Yes
TDGF1
Yes
THY1 (CD90)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
69XXY

Other Genotyping (Cell Line)