chESC-3 (H3)

General#

Cell Line

hPSCreg Name CSUe001-A
Alternative name(s)
chESC-3 (H3)
Cell line type Human embryonic stem cell (hESC)
Last update 16th April 2018
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Provider

Generator Central South University (CSU)
Derivation country China

External Databases

Cellosaurus CVCL_C063

General Information

Publications
Is the cell line available in principle?
No

Donor Information#

General Donor Information

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Culture Conditions#

Surface coating
Feeder cells Mouse embryonic fibroblast
Cellfinder Ont Id: EFO_0004040.
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
L-Glutamine
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)