ZZU-iPS-PD-RAB39b-001

General#

Cell Line

hPSCreg Name ZZUi005-A
Alternative name(s)
ZZU-iPS-PD-RAB39b-001
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 6th August 2017

Provider

Generator Zheng Zhou University - The first affiliated hospital (ZZU)
Owner Zheng Zhou University - The first affiliated hospital (ZZU)
Derivation country China

External Databases

BioSamples SAMEA104388410

General Information

Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male
Ethnicity Han

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Parkinson's disease
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Parkinson's syndrome
  • Parkinsons
  • Primary Parkinsonism
  • Parkinsons disease
  • Parkinson disease
  • Parkinson's disease (disorder)
  • Parkinson's disease NOS
  • Parkinson Disease, Idiopathic
  • PARKINSON DIS
  • Paralysis agitans
  • IDIOPATHIC PARKINSONS DIS
  • PARKINSON DIS IDIOPATHIC
  • Parkinsonism, Primary
  • Parkinson's Disease, Lewy Body
  • IDIOPATHIC PARKINSON DIS
  • Idiopathic PD
  • Idiopathic Parkinson Disease
  • Lewy Body Parkinson's Disease
  • Parkinsonian disorder
  • LEWY BODY PARKINSON DIS
  • Parkinson's
  • Idiopathic Parkinson's Disease
  • Parkinson's disease NOS (disorder)
  • Lewy Body Parkinson Disease
  • PARKINSONS DIS IDIOPATHIC
  • PARKINSONS DIS
  • Parkinson's Disease, Idiopathic
  • Parkinson syndrome
  • PARKINSONS DIS LEWY BODY
show more synonyms
Genetic variants
Xq28
NM_171998.3:c.536dupA
NP_741995.1:p.E179fsX48
Heterozygous
Disease associated phenotypes
  • calcification within the bilateral globus pallidus
  • intellectual disability
  • parkinsonism
  • extrapyramidal symptoms including tremor and rigidity
Family history The patient's uncle also suffer from this disease.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46XY
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104388411

Ethics#

We are currently revising the public data view for cell lines registered in hPSCreg. This section will be visible again in the next weeks.

hIPSC Derivation#

General

Primary cell type Fibroblast
Fibroblast in the OLS
Primary cell line name
Primary cell line vendor
Developmental stage primary cell Adult
Age of donor at collection 20-24
Date of collection 2016-12-22
Passage number reprogrammed P3

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Notes on reprogramming vector detection Reprogramming vector can't be detectable on passage 20.
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones These colonies will consist of iPSCs, which exhibit a hESC-like morphology characterized by a flatter cobblestone-like appearance with individual cells clearly demarcated from each other in the colonies.
Derived under xeno-free conditions
Yes
Derived under GMP?
Yes
Available as clinical grade?
No

Culture Conditions#

Surface coating Vitronectin
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 95 %
CO2 Concentration 5 %
Medium TeSR™ E8™
Supplements
20x supplement 25 ml
500x supplement 1 ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
Morphology pictures
AP staining of the cell line is positive.
Method documentation
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
GATA4
Yes
Protocol or reference
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
brachyury
Yes
Protocol or reference
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
TUBB3
Yes
Protocol or reference

Microbiology / Virus Screening

Certificate of Analysis

Is there a certificate of analysis available?
No

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Genetic Modifications (other than for reprogramming)