ESI-053

General#

Cell Line

hPSCreg Name SSCCe008-A
Alternative name(s)
ESI-053
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
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Provider

Generator Singapore Stem Cell Consortium (SSCC)
Derivation country Singapore

External Databases

Cellosaurus CVCL_B859
NIHhESC NIHhESC-11-0132

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics#

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
ZP removal technique Mechanical
Available as clinical grade?
Yes

Culture Conditions#

Passage method Enzymatically
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
Alkaline Phosphatase
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)