Generation of a transgene-free induced pluripotent stem cells line (UNIPDi002-A) from oral mucosa epithelial stem cells carrying the R304Q mutation in TP63 gene


Transgene free UNIPDi002-A-human induced pluripotent stem cell (hiPSC) line was generated by Sendai Virus Vectors reprogramming from human oral mucosal epithelial stem cells (hOMESCs) of a patient affected by ectrodactyly-ectodermal dysplasia-clefting (EEC)-syndrome, carrying a mutation in exon 8 of the TP63 gene (R304Q). The UNIPDi002-A-hiPSC line retained the mutation of the parental R304Q-hOMESCs and displayed a normal karyotype. No residual expression of transgenes nor Sendai virus vector sequences were detected in the line at passage 8. UNIPDi002-A-hiPSC expressed a panel of pluripotency-associated markers and could form embryoid bodies expressing markers belonging to the three germ layers ectoderm, endoderm and mesoderm. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Trevisan M, Barbaro V, Riccetti S, Masi G, Barzon L, Nespeca P, Alvisi G, Di Iorio E, Palù G
Journal Stem cell research
Publication Date 2018 Apr;28:149-152
PubMed 29486400
DOI 10.1016/j.scr.2018.02.006

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