TUSMi001-A
General#
Cell Line |
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hPSCreg Name | TUSMi001-A |
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 22nd May 2019 |
User feedback | |
Provider |
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Generator |
Tongji University School of Medicine (TUSM)
Contact:
Shanghai East Hospital (SHEH) |
Derivation country | China |
External Databases |
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BioSamples | SAMEA104381570 |
Cellosaurus | CVCL_LF70 |
General Information |
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Publications |
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* Is the cell line readily obtainable for third parties? |
Yes Research: allowed
Clinical: not allowed
Commercial: not allowed
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Donor Information#
General Donor Information |
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Sex | male |
Age of donor (at collection) | 55-59 |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Disease associated phenotypes | no phenotypes |
Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Yes
Karyotyping method:
G-Banding
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External Databases (Donor) |
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BioSamples | SAMEA104381571 |
Ethics#
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
Was the consent voluntarily given? | Yes |
Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
Provide contact information of the holder of the original Donor Information Sheet: | Yue Zhang, dyuezhang@126.com |
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? | No |
If you do not hold the SIGNED Donor Consent Form, do you know who does? | Yes |
Contact information / weblink | Yue Zhang, dyuezhang@126.com |
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? | Yes |
Alternatives to consent |
Alternative consent approval number |
Is there other documentation provided to the donor for consenting purposes? | No |
Confirm that consent was obtained by a qualified professional | Yes |
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
Details on restriction to research project |
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
How may genetic information associated with the cell line be accessed? | No information |
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
Please describe how access is provided: |
Contact data, institution, or website: |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
Name of accrediting authority involved? | Shanghai East Hospital Ethics Committee |
Approval number | EC.D(BG).016.01.1 |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
Name of accrediting authority involved? | Shanghai East Hospital Ethics Committee |
Approval number | EC.D(BG).016.01.1 |
Please describe: |
Further constraints on use |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
Constraints for use or distribution |
hIPSC Derivation#
General |
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Source cell type |
peripheral blood mononuclear cellA leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
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Age of donor (at collection) | 55-59 |
Passage number reprogrammed | 18 |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Episomal |
Genes | |
Is reprogramming vector detectable? |
Yes |
Methods used |
Immunostaining, PCR
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Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
No |
Derived under GMP? |
Yes |
Available as clinical grade? |
No |
Culture Conditions#
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
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O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
Score:
Marker | Present | Absent |
mCpG | ||
OCT4 |
Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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