Generation of a human control PBMC derived iPS cell line TUSMi001-A from a healthy male donor of Han Chinese genetic background
Summary
A 59-year old healthy man of Han Chinese genetic background donated his peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system was used to reprogram his PBMCs with the human OSKM (Oct4, Sox2, Kl4 and c-Myc) transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. In the studies of disease mechanism, the iPSC line can be used as a control. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Wang Y, Zhang Y, Zhang J, Lu J, Yang C, Zhao J, Li G, Liu Z, Lei Y |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2017 Dec;25:22-25 |
| PubMed | 29035843 |
| DOI | 10.1016/j.scr.2017.10.006 |