Generation of iPSC lines from peripheral blood mononuclear cells from five healthy donors
Summary
We describe the generation and characterisation of five human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. The iPSC lines exhibited a normal karyotype, and pluripotency was validated by flow cytometry and immunofluorescence of pluripotency markers, and their differentiation into cells representative of the three embryonic germ layers. These iPSC lines can be used as controls in studying disease mechanisms. Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Vlahos K, Sourris K, Yi Kuah J, Graham A, Suter A, Howden SE, Stanley EG, Elefanty AG |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Jun;69:103109 |
| PubMed | 37150143 |
| DOI | 10.1016/j.scr.2023.103109 |