M22c5, HSCC-003iPS-Sc5

General#

Cell Line

hPSCreg Name BCMi001-A
Alternative name(s)
M22c5, HSCC-003iPS-Sc5
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 1st June 2019
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Provider

Generator Baylor College of Medicine (BCM)
Owner Human Stem Cell Core (HSCC)
Distributors
Derivation country United States

External Databases

Cellosaurus CVCL_VT10
BioSamples SAMEA5843921

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Additional restrictions:

UBMTA must be signed between Baylor College of Medicine and recipient institution. A cell line transfer fee applies.

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 20-24
Ethnicity Caucasian, 22

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA5843922

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: +1-866-234-7673 / renee@zenbio.com
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? Yes
Contact information / weblink +1-866-234-7673 / http://www.zen-bio.com
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? Yes
Has the donor agreed to be re-contacted? Unknown
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Policy for access to genetic information No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Pearl IRB
Approval number 14-ZENB-101
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Thermo Fisher

hIPSC Derivation#

General

Source cell type
fibroblast
A connective-tissue cell of mesenchymal origin that secretes proteins and especially molecular collagen from which the extracellular fibrillar matrix of connective tissue forms.
Source cell origin
breast
The fore or ventral part of the body between the neck and the abdomen.
Age of donor (at collection) 20-24
Source cell line vendor Zen-Bio Inc.
Passage number reprogrammed 4

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Notes on reprogramming vector detection In the hPSC Scorecard, the SEV CT value was 40.

Vector free reprogramming

Other

Selection criteria for clones Clones were selected by morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
ReLeSR
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
38.94649 1.572758
Morphology pictures
Self-renewal
Unknown
Endoderm
Unknown
Mesoderm
Negative
Ectoderm score
Unknown
hPSC Scorecard Comparison of Undifferentiated vs. Embryoid Bodies (Day 26)
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Scorecard
Cardiac Muscle Cell
Ont Id: CL_0000746
In vitro directed differentiation
Scorecard
Neuron
Ont Id: CL_0000540
In vitro directed differentiation
Scorecard
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Scorecard

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)