M22c8, HSCC-003iPS-Sc8
The cell line is not validated yet.
BCMi001-B
General
Cell Line |
|
| hPSCreg name | BCMi001-B |
| Cite as: | BCMi001-B (RRID:CVCL_VT11) |
| Alternative name(s) |
M22c8, HSCC-003iPS-Sc8
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
BCMi001-A (M22c5, HSCC-003iPS-Sc5) |
| Last update | 18th August 2020 |
| User feedback | |
Provider |
|
| Generator | Baylor College of Medicine (BCM) |
| Owner | Baylor College of Medicine (BCM) |
| Distributors | |
| Derivation country | United States |
External Databases |
|
| Cellosaurus | CVCL_VT11 |
| BioSamples | SAMEA7333955 |
| Wikidata | Q93424747 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information
General Donor Information |
|
| Sex | male |
| Ethnicity | Caucasian, 22 |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA5843922 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Provide contact information of the holder of the original Donor Information Sheet: |
Renee Lea-Currie |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Contact information / weblink |
Renee Lea-Currie |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Pearl IRB |
| Approval number | 14-ZENB-101 |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type |
Synonyms
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| Passage number reprogrammed | 4 |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
Unknown |
Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Medium |
mTeSR™ 1
|
Characterisation
Analysis of Undifferentiated Cells
Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
qRT-PCR results for OCT4, SOX2, NANOG, and REX1 expression for 003iPS-Sc5 (BCMi001-A) and 003iPS-Sc8 (BCMi001-B). Bar graph shows fold-change over compared to the original fibroblasts.
Method documentation
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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