Generation of an induced pluripotent stem cell line from a healthy Caucasian male
Summary
The effects of genetic mutations on protein function can be studied in a physiologically relevant environment using tissue-specific cells differentiated from patient-derived induced pluripotent stem cells (iPSC). However, it is crucial to use iPSC derived from healthy individuals as control. We generated an iPS cell line from skin fibroblasts of a healthy Caucasian male by nucleofection of non-integrating episomal vectors. This cell line has normal karyotype, expresses pluripotency surface markers and pluripotency genes, and successfully differentiates into cells of the 3 germ layers. Therefore, it can be used as control for any disease of interest that is modelled using iPSC. Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Martínez-Moreno R, Pérez-Serra A, Carreras D, Aran B, Kuebler B, Brugada R, Scornik FS, Pérez GJ, Selga E |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 Apr;60:102717 |
| PubMed | 35231795 |
| DOI | 10.1016/j.scr.2022.102717 |