HPSI0214i-datg_2

General#

Cell Line

hPSCreg Name WTSIi048-A
Alternative name(s)
HPSI0214i-datg_2
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th May 2018
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Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors
Derivation country United Kingdom

External Databases

BioSamples SAMEA2632921
HipSci HPSI0214i-datg_2
Cellosaurus CVCL_AE49
ECACC 77650205
EBiSC WTSIi048-A

General Information

Publications
Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex female
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2398221
HipSci HPSI-datg

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 70-74
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
71.367 1.616

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 16

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0214i-datg_2
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 1
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ266959
Splice-aware STAR alignment
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368931
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203445
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861317
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448132
GATK haplotype calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1243472
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1203469
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369223
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267045
Abundances of transcripts
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266910
BWA alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266814
Imputed and phased genotypes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266720
mpileup variant calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203397
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1203429
Raw sequencing reads