SCIKFi001-A

D3-iR-2

General

Cell Line

hPSCreg Name
SCIKFi001-A
Alternative name(s)
D3-iR-2
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 25th April 2022
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Provider

Generator Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk. (SCIKF)
Owner Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk. (SCIKF)
Distributors
Derivation country Indonesia

External Databases

BioSamples SAMEA13371561
CLO CLO_0103780
Cellosaurus CVCL_C0J1
Wikidata Q112930350

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male
Ethnicity Asian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA13371560

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: linda.noviana@kalbe.co.id
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink linda.noviana@kalbe.co.id
Has the donor agreed to be re-contacted? Unknown
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? The Institutional Review Board, Stem Cell and Cancer Institute
Approval number 63/IRB/SCI/KF/REV/2015
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Mesenchymal Stem Cell
An undifferentiated stromal cell with the ability to develop into the cells that form distinct mesenchymal tissues; such as bone, muscle, connective tissue, blood vessels, and lymphatic tissue.
Synonyms
  • MPC
  • Mesenchymal Stem Cell
  • Mesenchymal Progenitor Cell
Source cell origin
Umbilical Cord
Extraembryonic structure that connects the fetus to the placenta. It contains two arteries and one vein.
Synonyms
  • Umbilical Cord
  • UMBILICAL CORD

Reprogramming method

Vector type Non-integrating
Vector self-replicating RNA
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA
mRNA

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Laminin
Feeder cells
No
Passage method Enzyme-free cell dissociation
ReLeSR
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
SOX2
Yes
SSEA-4
Yes
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal
Karyotyping method: G-Banding

Other Genotyping (Cell Line)