D3-iE
SCIKFi001-B
General
Cell Line |
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| hPSCreg name | SCIKFi001-B |
| Cite as: | SCIKFi001-B (RRID:CVCL_D0L0) |
| Alternative name(s) |
D3-iE
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
SCIKFi001-A (D3-iR-2) |
| Last update | 9th January 2024 |
| User feedback | |
Provider |
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| Generator | Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk. (SCIKF) |
| Owner | Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk. (SCIKF) |
| Derivation country | Indonesia |
External Databases |
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| Cellosaurus | CVCL_D0L0 |
| BioSamples | SAMEA115090065 |
| Wikidata | Q123033406 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Asian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Disease associated phenotypes | no phenotypes |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
46XY
Karyotyping method:
G-Banding
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA13371560 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Provide contact information of the holder of the original Donor Information Sheet: | linda.noviana@bifarma.co.id |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Contact information / weblink | linda.noviana@bifarma.co.id |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent development of commercial products? | No |
| Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
Does consent permit research by | |
| an academic institution? | Yes |
| a public organisation? | Yes |
| a non-profit company? | Yes |
| a for-profit corporation? | No |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Stem Cell and Cancer Institute Institutional Review Board |
| Approval number | 63/IRB/SCI/KF/REV/2015 |
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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| Source cell type |
A mesenchymal stem cell that is part of Wharton's jelly.
|
| Source cell origin |
The connecting cord from the developing embryo to the placenta.; See notes for connecting stalk
Synonyms
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Laminin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
|
| Medium |
mTeSR™ 1
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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Morphology pictures
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46,XY,der(21)t(5;21)(q12;q22)
Passage number: 17
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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