Generation of induced pluripotent stem cell line RCPCMi004-A derived from patient with Parkinson's disease with deletion of the exon 2 in PARK2 gene
Summary
IPSC line RCPCMi004-A was generated from skin fibroblasts collected from a male patient with early onset Parkinson's disease. The patient carries a heterozygous deletion of the exon 2 of PARK2 gene. The reprogramming of fibroblasts was performed with Sendai viruses containing Oct-4, Sox-2, Klf-4 and c-Myc. Pluripotency was confirmed by immunofluorescence, RT-PCR, and formation of embryoid bodies. The RCPCMi004-A cell line carries the same deletion in PARK2 gene. The RCPCMi004-A cell line can be used to model Parkinson's disease in vitro. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Shuvalova LD, Eremeev AV, Bogomazova AN, Novosadova EV, Zerkalenkova EA, Olshanskaya YV, Fedotova EY, Glagoleva ES, Illarioshkin SN, Lebedeva OS, Lagarkova MA |
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| Journal | Stem cell research |
| Publication Date | 2020 Apr;44:101733 |
| PubMed | 32151951 |
| DOI | 10.1016/j.scr.2020.101733 |