Generation of induced pluripotent stem cell line RCPCMi004-A derived from patient with Parkinson's disease with deletion of the exon 2 in PARK2 gene


IPSC line RCPCMi004-A was generated from skin fibroblasts collected from a male patient with early onset Parkinson's disease. The patient carries a heterozygous deletion of the exon 2 of PARK2 gene. The reprogramming of fibroblasts was performed with Sendai viruses containing Oct-4, Sox-2, Klf-4 and c-Myc. Pluripotency was confirmed by immunofluorescence, RT-PCR, and formation of embryoid bodies. The RCPCMi004-A cell line carries the same deletion in PARK2 gene. The RCPCMi004-A cell line can be used to model Parkinson's disease in vitro. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Shuvalova LD, Eremeev AV, Bogomazova AN, Novosadova EV, Zerkalenkova EA, Olshanskaya YV, Fedotova EY, Glagoleva ES, Illarioshkin SN, Lebedeva OS, Lagarkova MA
Journal Stem cell research
Publication Date 2020 Apr;44:101733
PubMed 32151951
DOI 10.1016/j.scr.2020.101733

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