SDQLCHi061-A

General

Cell Line

hPSCreg name SDQLCHi061-A
Cite as:
SDQLCHi061-A (RRID:CVCL_C9JL)
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 31st January 2024
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Provider

Generator Children’s Hospital affiliated to Shandong University (SDQLCH)

External Databases

Cellosaurus CVCL_C9JL
BioSamples SAMEA115084979
Wikidata Q123033471

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • CPS I deficiency
  • CPS1 deficiency
  • CPS1D
  • carbamoyl phosphate synthetase I deficiency disease
  • carbamoyl phosphate synthetase deficiency
  • carbamoyl-phosphate synthetase I deficiency
  • carbamoyl-phosphate synthetase deficiency
  • carbamoylphosphate synthetase I deficiency
  • CPS 1 deficiency
  • carbamoyl phosphate synthetase 1 deficiency
  • carbamoyl phosphate synthetase 1 deficiency, hyperammonemia due to
  • carbamoyl phosphate synthetase I deficiency, hyperammonemia due to
  • carbamoyl-phosphate synthetase 1 deficiency
  • carbamyl phosphate synthetase (CPS) deficiency
  • hyperammonemia due to carbamoyl phosphate synthetase 1 deficiency
  • carbamoyl-phosphate synthase deficiency disease
show more synonyms

External Databases (Donor)

BioSamples SAMEA115084980

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Medical Ethics Committee of Children’s Hospital Affiliated to Shandong University
Approval number QLET-IRB/P-2021005
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC
Source cell origin
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Other medium:
Base medium: hiPSC/hESC medium-ncTarget
Main protein source:
Serum concentration: %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SOX2
Yes
NANOG
Yes
TRA 1-60
Yes
Marker Present Absent
mCpG
OCT4
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
HAND1
Yes
TBXT
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes
SOX1
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Passage number: 10
Karyotyping method: G-Banding

Other Genotyping (Cell Line)